The occurrence of methylated guanines in ribonucleic acids from several sources.
نویسندگان
چکیده
A number of additional purines and pyrimidines have recently been described as minor constituents of ribonucleic acids. Cohn (1957, 1958, 1959) and Davis & Allen (1957) have described an unusual pyrimidine nucleotide from yeast ribonucleic acid, thymine, 2-methyladenine, 6-methylaninopurine and 6-dimethylaminopurine have been identified as components ofribonucleic acid from several sources (Littlefield & Dunn, 1958a, b), and 5-methylcytosine has been detected in ribonucleic acid from Eacherichia coli (Amos & Korn, 1958) and from wheat embryo, liver and Aerobacter aerogenes (Dunn, unpublished work). The occurrence of methylated adenines in ribonucleic acid led us to look for methyl-substituted guanines. We have found 1methylguanine, 6-hydroxy-2-methylaminopurine and 2-dimethylamino-6-hydroxypurine to be constituents of ribonucleic acid from several different biological sources, by identification of the free bases, ribonucleosides and ribonucleotides from the ribonucleic acids. While this work was in progress Adler, Weissmann & Gutman (1958) reported the isolation of two of these bases, 1-methylguanine and 6-hydroxy-2-methylaminopurine from an acid hydrolysate of yeast ribonucleic acid. Preliminary results of our work have been reported (Dunn & Smith, 1959a, b).
منابع مشابه
The occurrence and distribution of thymine and three methylated-adenine bases in ribonucleic acids from several sources.
It has been known for several years that deoxyribonucleic acid may normally contain 5-methylcytosine (Wyatt, 1951), 5-hydroxymethylcytosine (Wyatt & Cohen, 1953), and 6-methylaminopurine (Dunn & Smith, 1955, 1958a) in addition to adenine, guanine, cytosine and thymine. The first evidence of such additional bases naturally present in ribonucleic acid is the recent isolation of an unidentified nu...
متن کاملOccurrence of Methylated Purine Bases in Yeast Ribonucleic Acid*
A number of methylated purine bases have recently been reported to occur in small quantities in the urine of normal human subjects. These include 1-methylguanine, iV*-methylguanine (6-hydroxy-2-methylaminopurine) ,I 1-methylhypoxanthine, and 8-hydroxy-7-methylguanine (1) in addition to 7-methylguanine, previously known as a urinary constituent (2). These compounds were found to be metabolites o...
متن کاملOccurrence and distribution of methylated purines in the ribonucleic acids of subcellular fractions.
متن کامل
Occurrence of methylated purine bases in yeast ribonucleic acid.
A number of methylated purine bases have recently been reported to occur in small quantities in the urine of normal human subjects. These include 1-methylguanine, iV*-methylguanine (6-hydroxy-2-methylaminopurine) ,I 1-methylhypoxanthine, and 8-hydroxy-7-methylguanine (1) in addition to 7-methylguanine, previously known as a urinary constituent (2). These compounds were found to be metabolites o...
متن کاملPattern of DNA cytosine methylation in Aeluropus littoralis during temperature stress
DNA methylation as an epigenetic mediator plays the important role in spatial and temporal gene regulation and ensures the stability and the plasticity of organism. In this investigation, methylation sensitive amplification polymorphism (MSAP) were assessed in CCGG sites on a halophytic plant, Aeluropuslittoralis in response to different temperature stresses including freezing...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Biochemical journal
دوره 72 2 شماره
صفحات -
تاریخ انتشار 1959